Purification and Kinetics of Bovine Kidney Cortex Glutathione Reductase

Author(s): Berivan Tandogan, N. Nuray Ulusu

Journal Name: Protein & Peptide Letters

Volume 17 , Issue 5 , 2010


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Abstract:

Glutathione reductase was purified 34806-fold with a final yield of 85 % from the bovine kidney cortex. Some molecular and kinetic properties of purified enzyme are investigated. Product inhibition studies showed that the enzyme obeys ‘branched’ mechanism: KmNADPH 18 ± 3 μM and KmGSSG 65 ± 5 μM were determined.

Keywords: Glutathione reductase, purification, kinetics, branched mechanism

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Article Details

VOLUME: 17
ISSUE: 5
Year: 2010
Page: [667 - 674]
Pages: 8
DOI: 10.2174/092986610791112684
Price: $65

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