Stabilization of Folding Intermediate States from Alkaline Induced Unfolded State of Bovine Serum Fetuin in Trifluoroethanol and Acetonitrile

Author(s): Basir Ahmad, Zeyaul Islam, Ankita Varshney, Rizwan Hasan Khan

Journal Name: Protein & Peptide Letters

Volume 17 , Issue 5 , 2010

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Abstract:

The conformation of bovine serum fetuin (BSF) was examined over the pH 7.0-12.9 regions by circular dichroism, intrinsic fluorescence and ANS binding. We observed that at higher pH, BSF exists in alkaline unfolded state. Our results provided evidence that correlates simultaneous formation of secondary structure followed by accumulation of hydrophobic clusters.

Keywords: Acetonitrile, alkali denaturation, bovine serum fetuin, circular dichroism, trifluoroethanol

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Article Details

VOLUME: 17
ISSUE: 5
Year: 2010
Page: [660 - 666]
Pages: 7
DOI: 10.2174/092986610791112774
Price: $65

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