Identification of the Amino Acid Residues Affecting the Catalytic Pocket of the Sulfolobus solfataricus Signature Amidase

Title: Identification of the Amino Acid Residues Affecting the Catalytic Pocket of the Sulfolobus solfataricus Signature Amidase

Volume: 17 Issue: 2

Author(s): Cilia Elisa and Ammendola Sergio

Affiliation:

Keywords: Functional residues, amidase, machine learning, SVM, FRAP

Abstract: 36 mutants of the Sulfolobus solfataricus amidase were analyzed by comparing biochemical data to structural data obtained by a learning machine. The analysis shows that beside well known catalytic residues, amino acid residues Arg197, Lys209 and Asp228 are important for the catalytic activity of the signature thermophilic amidase.

Cite this article as:

Elisa Cilia and Sergio Ammendola, Identification of the Amino Acid Residues Affecting the Catalytic Pocket of the Sulfolobus solfataricus Signature Amidase, Protein & Peptide Letters 2010; 17 (2) . https://dx.doi.org/10.2174/092986610790226021