Identification of a Cell-Bound Extracellular Protease Overproduced by Sulfolobus solfataricus in Peptide-Rich Media

Author(s): Raffaele Cannio, Giuliana Catara, Imma Fiume, Marco Balestrieri, Mose Rossi, Gianna Palmieri

Journal Name: Protein & Peptide Letters

Volume 17 , Issue 1 , 2010

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A new protease, named SsMTP was identified from the archeon Sulfolobus solfataricus. The enzyme is associated to the cell-membrane and over-produced in response to the peptide-enriched media. SsMTP has a molecular mass of 120 kDa showing optimal activity at pH 2.0 in the temperature range 70 - 90 °C, and a half-life of 20 days at 80 °C. Primary structure analysis revealed that SsMTP represents a novel type of multi-domain thermopsin-like protease containing the catalytic domain followed by two distinct domains, PKD and Y_Y_Y, which are usually involved in a range of protein- protein interactions among the extracellular proteins.

Keywords: Sulfolobus solfataricus, Membrane-bound extracellular protease, Thermopsin-like protease, Multi-domain protease

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Article Details

Year: 2010
Page: [78 - 85]
Pages: 8
DOI: 10.2174/092986610789909458

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