Invertase from Hyper Producer Strain of Aspergillus niger: Physiochemical Properties, Thermodynamics and Active Site Residues Heat of Ionization

Author(s): Habibullah Nadeem, Muhammad Hamid Rashid, Muhammad Riaz, Bibi Asma, Muhammad Rizwan Javed, Raheela Perveen

Journal Name: Protein & Peptide Letters

Volume 16 , Issue 9 , 2009


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Abstract:

Here we report for the first time heat of ionization of invertase (E.C.3.2.1.26) active site residues from hyperproducer strain of Aspergillus niger (34.1 U ml-1), along with its physiochemical properties, kinetics and thermodynamics of stability-function. The Invertase showed great potential for industry as being highly efficient (kcat = 24167 s-1 at 65 °C, pH 5.0) and stable (half life= 12 h at 56°C).

Keywords: Thermostability, Enthalpy, Entropy, Free energy, Ionization energy

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Article Details

VOLUME: 16
ISSUE: 9
Year: 2009
Page: [1098 - 1105]
Pages: 8
DOI: 10.2174/092986609789055322
Price: $65

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