The purpose of this study was to evaluate the efficacy of newly synthesized bibenzoimidazolyl derivatives 2(ai) as antimicrobials. These molecules are obtained by the reaction of different pharmaceutically important bioactive aralkyl halides with 1, 7-dimethyl-2-propyl-1H, 3H-[2, 5]bibenzoimidazole in presence of powdered potassium carbonate in DMF solvent under both conventional and microwave methods. The microwave method offers the products 2(a-i) in higher yields compared to conventional method. All the synthesized compounds were characterized by IR, 1H NMR and elemental analysis and evaluated their efficacy as antimicrobials. We observed the significant differences between the antimicrobial activities of the newly synthesized compounds based on their various substituents present at N1 position. Compounds 2h, 2a, 2c, 2e and 2f showed potent antimicrobial activity compared to standard drugs.