Title: Mitochondria-Targeted Green Fluorescent Protein for Quantitative Monitoring of Mitochondrial Morphology in Living Cells
VOLUME: 6 ISSUE: 5
Author(s):Yasutomo Nomura, Toshifumi Takayama, Michihiko Sato, Eiji Takahashi, Zhonggang Feng and Takao Nakamura
Affiliation:Department of Environmental Life Science, Graduate School of Medical Science, Yamagata University, Yonezawa 992-8510, Japan.
Keywords:Mitochondria, GFP, RH123, Image processing, Photobleaching
Abstract: In addition to Rhodamine 123, a progress in genetic engineering allowed the microscopic observation of mitochondria with GFP (mtGFP). Here we evaluated these labeling procedures for their ability to analyze mitochondrial morphology quantitatively. Expression of mtGFP was the optimal procedure to monitor mitochondrial morphology quantitatively, because of photo-stability and precise localization.
