Purification and Characterization of Human Brain Serine Racemase Expressed in Moderately Halophilic Bacteria

Author(s): Chizuru Nagayoshi, Matsujiro Ishibashi, Masao Tokunaga

Journal Name: Protein & Peptide Letters

Volume 16 , Issue 2 , 2009


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Abstract:

We have successfully expressed an active human brain serine racemase (hSR) with His-tag using moderate halophile. The purified His-hSR showed high elimination and racemization activities on L-serine: the elimination activity was 2.6-fold higher than racemization activity. Both enzyme activities showed an optimum reaction pH at around 9.0 and were stimulated 5- to 7-fold by such divalent cations as Mg++, Mn++ and Ca++.

Keywords: Human serine racemase, porin, groE, His-tag, halophile

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Article Details

VOLUME: 16
ISSUE: 2
Year: 2009
Page: [201 - 206]
Pages: 6
DOI: 10.2174/092986609787316261
Price: $65

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