The Inhibitory Effect of Propofol on Bovine Lactoperoxidase

Author(s): Melda Sisecioglu, Murat Cankaya, Ilhami Gulcin, Hasan Ozdemir

Journal Name: Protein & Peptide Letters

Volume 16 , Issue 1 , 2009


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Abstract:

Propofol (2,6-diisopropylphenol) is a hypnotic intravenous agent with in vivo antioxidant properties. This study was undertaken to examine the in vitro effect of propofol on lactoperoxidase (LPO; E.C. 1.11.1.7) obtained from bovine milk. Lactoperoxidase was purified with three purification steps: Amberlite CG-50 resin, CM-Sephadex C-50 ionexchange chromatography and Sephadex G-100 gel filtration chromatography, respectively. Lactoperoxidase was purified with a yield of 21.6%, a specific activity of 34 EU/mg proteins and 14.7-fold purification. One enzyme unit is defined as the oxidation of 1 μmol ABTS per min under the assay condition (25oC, pH: 6.0). To determine enzyme purity, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed and single band was observed. The effect of propofol on lactoperoxidase were determined using 2,2-azino-bis (3-ethylbenzthiazoline-6 sulfonic acid) diammonium salt (ABTS) as a chromogenic substrate. The IC50 value of propofol was found as 15.97 μM. Also, Ki constant for propofol was 3.72 μM and propofol was found as competitive inhibitor.

Keywords: Propofol, lactoperoxidase, enzyme, purification, inhibition, chromatography

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Article Details

VOLUME: 16
ISSUE: 1
Year: 2009
Page: [46 - 49]
Pages: 4
DOI: 10.2174/092986609787049394

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