Construction, Expression, Purification and Immunology Effect of an Antiatherosclerosis Chimeric Enzyme Vaccine in Escherichia coli

Author(s): Sun Yunxiao, Li Zhufang, Yang Xin, Long Jun, Xiong Qiyan, Qi Gaofu, Cao Rongyue, Wu Jie, Liu Jingjing, Fan Hao, Li Taiming

Journal Name: Protein & Peptide Letters

Volume 15 , Issue 7 , 2008


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Abstract:

In order to prevent atherosclerosis, a chimeric enzyme vaccine of AnsB-TTP-PADRE-CETPC was successfully constructed, expressed and purified to immunize New Zealand white rabbits for inducing high titers of anti-CETP antibodies to improve lipid abnormality. The protein was expressed as soluble protein in Escherichia coli and purified by anion exchange column and Sephadex G-100 size-exclusion chromatography. After immunizing rabbits with the purified protein, high titer anti-CETP antibodies were induced and lasted more than nineteen weeks in vivo; High density lipoprotein cholesterol (HDL-C) content in the serum was elevated to 61% while decreased low density lipoprotein cholesterol (LDL-C) to 37.2% compared with control rabbits in the presence of Al(OH)3.

Keywords: Atherosclerosis, vaccine, CETP B cell epitope, antibody, construction, expression and purification

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Article Details

VOLUME: 15
ISSUE: 7
Year: 2008
Page: [745 - 752]
Pages: 8
DOI: 10.2174/092986608785133654
Price: $65

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