Characterization of Mutants of Sulfolobus solfataricus Signature Amidase Able to Hydrolyse R-Ketoprofen Amide

Author(s): Cesare Giordano, Sergio Ammendola

Journal Name: Protein & Peptide Letters

Volume 15 , Issue 6 , 2008


Become EABM
Become Reviewer
Call for Editor

Abstract:

The amidase from Sulfolobus solfataricus enantioselectively hydrolyzes S-ketoprofen amide to its corresponding acid. We identify three independent SsAH mutants that hydrolyze R-ketoprofen amide and built computational models of their three-dimensional structure. Interestingly the mutations do not specifically affect residues near the active site, or directly interacting with the substrate.

Keywords: Enantioselectivity, Amidase, Mutation, Modelling, R-Ketoprofen

Rights & PermissionsPrintExport Cite as

Article Details

VOLUME: 15
ISSUE: 6
Year: 2008
Published on: 01 March, 2012
Page: [617 - 623]
Pages: 7
DOI: 10.2174/092986608784966949
Price: $65

Article Metrics

PDF: 5