Protein Preparation, Crystallization and Preliminary X-Ray Crystallographic Analysis of N-Acetylglutamate Kinase from Streptococcus mutans

Author(s): Chang-Wei Wu, Lan-Fen Li, Xiang Liu, Xiong-Zhuo Gao, Jian Lei, Xiao-Dong Su, Xiaojun Zhao, Yu-He Liang

Journal Name: Protein & Peptide Letters

Volume 15 , Issue 5 , 2008

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Abstract:

The N-acetylglutamate kinase from Streptococcus mutans was expressed in Escherichia coli in soluble form and purified to homogeneity. Crystals suitable for X-ray diffraction were obtained by hanging-drop vapor diffusion method and diffracted to 2.06 Å. The crystal belonged to space group P21212, with unit cell parameters a = 57.19 Å, b =94.76 Å, c =47.58 Å. The gel filtration and initial phasing results showed that the enzyme exists as a monomer, which is different from previously reported N-acetylglutamate kinases.

Keywords: Streptococcus mutans, N-acetylglutamate kinase (NAGK), arginine biosynthesis, protein crystallography

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Article Details

VOLUME: 15
ISSUE: 5
Year: 2008
Page: [541 - 543]
Pages: 3
DOI: 10.2174/092986608784567519
Price: $65

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