Crystallization and Preliminary X-Ray Diffraction Analysis of a Novel Mannose-Binding Lectin with Antiretroviral Properties from Polygonatum cyrtonema Hua

Author(s): Jing-Jin Ding, Jin-ku Bao, De-Yu Zhu, Ying Zhang, Da-Cheng Wang

Journal Name: Protein & Peptide Letters

Volume 15 , Issue 4 , 2008

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Abstract:

A novel antiretroviral protein Polygonatum cyrtonema lectin (PCL) belonging to the monocot mannose-binding lectin (MMBL) superfamily has been crystallized using hanging-drop vapor-diffusion method. The crystals diffract to 2.0 Å resolution and belong to space group P21, with unit-cell parameters of a=39.308 Å, b=48.317 Å, c=112.221 Å, and β=90.12° . Preliminary analysis indicates that the asymmetric unit contains four PCL molecules with a solvent content of about 45%. A set of X-ray data has been collected for the crystal structure determination.

Keywords: PCL, monocot mannose-binding lectin, crystallization, preliminary X-ray diffraction analysis, Polygonatum cyrtonema Hua

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Article Details

VOLUME: 15
ISSUE: 4
Year: 2008
Page: [411 - 414]
Pages: 4
DOI: 10.2174/092986608784246551
Price: $65

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