Expression of a Canavalia brasiliensis Lectin (ConBr) Precursor in Pichia pastoris

Author(s): C. P.S. Carvalho, C. S. Rocha, D. R. Nepomuceno, J. T.A. Oliveira, T. B. Grangeiro

Journal Name: Protein & Peptide Letters

Volume 15 , Issue 4 , 2008

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A precursor of ConBr, a glucose/mannose-binding plant lectin, was expressed in the yeast Pichia pastoris. Western blot analysis of transformed cells detected an intracellularly recombinant protein band with ca. 34.5 kDa. The recombinant protein was apparently active as suggested by its strong interaction with the mannose-rich yeast cell debris.

Keywords: Recombinant protein, carbohydrate-binding, phytohemagglutinin, yeast

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Article Details

Year: 2008
Page: [327 - 332]
Pages: 6
DOI: 10.2174/092986608784246579
Price: $65

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