Abstract
A precursor of ConBr, a glucose/mannose-binding plant lectin, was expressed in the yeast Pichia pastoris. Western blot analysis of transformed cells detected an intracellularly recombinant protein band with ca. 34.5 kDa. The recombinant protein was apparently active as suggested by its strong interaction with the mannose-rich yeast cell debris.
Keywords: Recombinant protein, carbohydrate-binding, phytohemagglutinin, yeast
Protein & Peptide Letters
Title: Expression of a Canavalia brasiliensis Lectin (ConBr) Precursor in Pichia pastoris
Volume: 15 Issue: 4
Author(s): C. P.S. Carvalho, C. S. Rocha, D. R. Nepomuceno, J. T.A. Oliveira and T. B. Grangeiro
Affiliation:
Keywords: Recombinant protein, carbohydrate-binding, phytohemagglutinin, yeast
Abstract: A precursor of ConBr, a glucose/mannose-binding plant lectin, was expressed in the yeast Pichia pastoris. Western blot analysis of transformed cells detected an intracellularly recombinant protein band with ca. 34.5 kDa. The recombinant protein was apparently active as suggested by its strong interaction with the mannose-rich yeast cell debris.
Export Options
About this article
Cite this article as:
Carvalho P.S. C., Rocha S. C., Nepomuceno R. D., Oliveira T.A. J. and Grangeiro B. T., Expression of a Canavalia brasiliensis Lectin (ConBr) Precursor in Pichia pastoris, Protein & Peptide Letters 2008; 15 (4) . https://dx.doi.org/10.2174/092986608784246579
DOI https://dx.doi.org/10.2174/092986608784246579 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
The BET-Bromodomain Inhibitor JQ1 Reduces Inflammation and Tau Phosphorylation at Ser396 in the Brain of the 3xTg Model of Alzheimer’s Disease
Current Alzheimer Research Reduction of Genotoxicity of Carbamazepine to Human Lymphocytes by Pre-treatment with Vitamin B12
Current Molecular Pharmacology Protein-Directed Immobilization of Phosphocholine Ligands on a Gold Surface for Multivalent C-Reactive Protein Binding
Current Topics in Medicinal Chemistry The Role of Berberine in the Multi-Target Treatment of Senile Dementia
Current Topics in Medicinal Chemistry Editorial [Hot Topic: Osteoarthritis and Its Treatment (Guest Editor: Demitrios H. Vynios)]
Current Medicinal Chemistry - Anti-Inflammatory & Anti-Allergy Agents Modified LDL Immune Complexes and Cardiovascular Disease
Current Medicinal Chemistry Protein C Inhibitor as an Anti-Disseminated Intravascular Coagulation Agent - Mechanism and Modification
Current Medicinal Chemistry - Cardiovascular & Hematological Agents Predicting Targeted Polypharmacology for Drug Repositioning and Multi- Target Drug Discovery
Current Medicinal Chemistry Novel Therapies Against Aggressive and Recurrent Epithelial Cancers by Molecular Targeting Tumor- and Metastasis-Initiating Cells and Their Progenies
Anti-Cancer Agents in Medicinal Chemistry CXCR3-binding Chemokines: Novel Multifunctional Therapeutic Targets
Current Drug Targets - Immune, Endocrine & Metabolic Disorders An Insight into Molecular Mechanisms and Novel Therapeutic Approaches in Epileptogenesis
CNS & Neurological Disorders - Drug Targets Carbon Nanotubes in the Diagnosis and Treatment of Malignant Melanoma
Anti-Cancer Agents in Medicinal Chemistry Probiotic Research in Australia, New Zealand and the Asia-Pacific Region
Current Pharmaceutical Design Mitochondrial Dysfunction and Antioxidant Therapy in Sepsis
Infectious Disorders - Drug Targets The Roles of ID, IA and IK in the Electrophysiological Functions of Small-Diameter Rat Trigeminal Ganglion Neurons
Current Molecular Pharmacology Clinical Considerations in Developing Dendritic Cell Vaccine Based Immunotherapy Protocols in Cancer
Current Molecular Medicine Melatonin-Immune System Relationships
Current Topics in Medicinal Chemistry T-Lymphocytes: A Target for Stimulatory and Inhibitory Effects of Zinc Ions
Endocrine, Metabolic & Immune Disorders - Drug Targets Dysregulated NF-κB Pathway in Peripheral Mononuclear Cells of Alzheimers Disease Patients
Current Alzheimer Research TLR Signaling Pathways: Opportunities for Activation and Blockade in Pursuit of Therapy
Current Pharmaceutical Design