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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Expression of a Canavalia brasiliensis Lectin (ConBr) Precursor in Pichia pastoris

Author(s): C. P.S. Carvalho, C. S. Rocha, D. R. Nepomuceno, J. T.A. Oliveira and T. B. Grangeiro

Volume 15, Issue 4, 2008

Page: [327 - 332] Pages: 6

DOI: 10.2174/092986608784246579

Price: $65

Abstract

A precursor of ConBr, a glucose/mannose-binding plant lectin, was expressed in the yeast Pichia pastoris. Western blot analysis of transformed cells detected an intracellularly recombinant protein band with ca. 34.5 kDa. The recombinant protein was apparently active as suggested by its strong interaction with the mannose-rich yeast cell debris.

Keywords: Recombinant protein, carbohydrate-binding, phytohemagglutinin, yeast


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