Stabilization of Beta-Propeller Phytase by Introducing Xaa→Pro and Gly→Ala Substitutions at Consensus Positions

Title: Stabilization of Beta-Propeller Phytase by Introducing Xaa→Pro and Gly→Ala Substitutions at Consensus Positions

Volume: 15 Issue: 3

Author(s): Kam-Bo Wong, Edward T.K. Tung, Hoi-Wah Ma, Chiwai Cheng and Boon L. Lim

Affiliation:

Keywords: Beta-propeller phytase, protein engineering, proline, unfolding entropy, protein stability

Abstract: Phytases play important roles in agricultural and feed industries. In this study, the stability of a beta-propeller phytase, PhyL, from Bacillus licheniformis was successfully improved by introducing Xaa→Pro and Gly→Ala substitutions at consensus positions. Our results suggest that Gly→Ala substitution is a more promising strategy to improve protein stability.

Cite this article as:

Wong Kam-Bo, Tung T.K. Edward, Ma Hoi-Wah, Cheng Chiwai and Lim L. Boon, Stabilization of Beta-Propeller Phytase by Introducing Xaa→Pro and Gly→Ala Substitutions at Consensus Positions, Protein & Peptide Letters 2008; 15 (3) . https://dx.doi.org/10.2174/092986608783744216