Stabilization of Beta-Propeller Phytase by Introducing Xaa→Pro and Gly→Ala Substitutions at Consensus Positions

Author(s): Kam-Bo Wong, Edward T.K. Tung, Hoi-Wah Ma, Chiwai Cheng, Boon L. Lim

Journal Name: Protein & Peptide Letters

Volume 15 , Issue 3 , 2008

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Abstract:

Phytases play important roles in agricultural and feed industries. In this study, the stability of a beta-propeller phytase, PhyL, from Bacillus licheniformis was successfully improved by introducing Xaa→Pro and Gly→Ala substitutions at consensus positions. Our results suggest that Gly→Ala substitution is a more promising strategy to improve protein stability.

Keywords: Beta-propeller phytase, protein engineering, proline, unfolding entropy, protein stability

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Article Details

VOLUME: 15
ISSUE: 3
Year: 2008
Page: [297 - 299]
Pages: 3
DOI: 10.2174/092986608783744216
Price: $65

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