Mycobacterium Tuberculosis HSP16.3 is a major antigen maximally expressed during the stationary phase. Previous studies showed that HSP16.3 can function as a molecular chaperone in vitro. Here, crystallization trails of HSP 16.3 were reported. A kind of crystal can be diffracted to 2.8 A resolution at the ”Photon Factory“, a synchrotron light source in Japan. The crystal displayed the space group R3 with unit cell parameters a=b=110 A , c=152 A and gamma=120 degree. Assuming the presence of 9 HSP16.3 molecules in an asymmetric unit, it gives a Vm= 0.73 A 3 / Da. and solvent content of 35percent by volume.
Keywords: MYCOBACTERIUM TUBERCULOSIS HSP16.3, IPTG isopropyl ß-D-thiogalacto-pyranoside, MPD 2-Methyl-2,4-Pentanediol, sHSP small heat shock protein, MAD Multiwavelength Anomalous Dispersion, molecular chaperone activity
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