Abstract
Venoms from Bothrops jararcussu Bothrops asper Bothrops atrox Bothrops pirajai Bothrops moojeni Bothrops alternatus and Bothrops (Bothriopasis) bilineata were fractionated using a simplified procedure based on-exchange chromatography on CM-Sepharose at pH 8.0 or reverse phase HPLC.The resulting elution profilesshowed important differences in the myotoxin contentof these venoms.The venoms froms B.alternatus,B.atrox and Bothriopsis bilineata did not contain the major myotoxin found in the other venoms.The amino acid sequences of the first 50 residues of the N-terminal region of the PLA2-Like mytoxins showed a homology of 90-96percent with other bothropic myotoxins.All of the myotoxins isolated induced rat paw edema, increased the level of plasma ceratine kinase and produced myonecrosis together with polymorphoniclear cell infiltration.
Keywords: Myotoxic Phospholipase A2, Bothrops Venom, Bothrops jararcussu, Bothrops asper, Bothrops atrox, Bothrops pirajai, Bothrops moojeni, Bothrops alternatus, Bothrops Bothriopsis, blineata
Protein & Peptide Letters
Title: Comparative Biochemical Studies of Myotoxic Phospholipase A2 From Bothrops Venom
Volume: 8 Issue: 3
Author(s): M. H. Toyama, A. M. Soares, S. H. Andriao-Escarso, J. C. Novello, B. Oliveira, J. R. Giglio, M. R.M. Fontes and S. Marangoni
Affiliation:
Keywords: Myotoxic Phospholipase A2, Bothrops Venom, Bothrops jararcussu, Bothrops asper, Bothrops atrox, Bothrops pirajai, Bothrops moojeni, Bothrops alternatus, Bothrops Bothriopsis, blineata
Abstract: Venoms from Bothrops jararcussu Bothrops asper Bothrops atrox Bothrops pirajai Bothrops moojeni Bothrops alternatus and Bothrops (Bothriopasis) bilineata were fractionated using a simplified procedure based on-exchange chromatography on CM-Sepharose at pH 8.0 or reverse phase HPLC.The resulting elution profilesshowed important differences in the myotoxin contentof these venoms.The venoms froms B.alternatus,B.atrox and Bothriopsis bilineata did not contain the major myotoxin found in the other venoms.The amino acid sequences of the first 50 residues of the N-terminal region of the PLA2-Like mytoxins showed a homology of 90-96percent with other bothropic myotoxins.All of the myotoxins isolated induced rat paw edema, increased the level of plasma ceratine kinase and produced myonecrosis together with polymorphoniclear cell infiltration.
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Cite this article as:
Toyama H. M., Soares M. A., Andriao-Escarso H. S., Novello C. J., Oliveira B., Giglio R. J., Fontes R.M. M. and Marangoni S., Comparative Biochemical Studies of Myotoxic Phospholipase A2 From Bothrops Venom, Protein & Peptide Letters 2001; 8 (3) . https://dx.doi.org/10.2174/0929866013409445
DOI https://dx.doi.org/10.2174/0929866013409445 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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