Title: Crystallization and Preliminary X-Ray Crystallographic Studies of Human Autocrine Motility Factor
VOLUME: 8 ISSUE: 4
Author(s):Hiroshi Uemura, Nobutada Tanaka, Tatsuyshi Funasaka, Arayo Haga, Hisamitsu Nagase, Avraham Raz and Kazuo T. Nakamura
Affiliation:School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, JAPAN
Keywords:Crystallization, Motility Factor, cytokine
Abstract: Human autocrine motility factor (hAMF), a tumor-secreted cytokine which stimulates cell migration in vitro and metastasis in vivo, has been crystallized by the hanging-drop vapour diffusion method. The crystals belong to an orthorhombic space group P212121 with the cell dimensions of a = 80.79 A, b = 107.1 A, and c = 270.9 A. There are two dimers per asymmetric unit. The crystals diffract to at least 2.0 A resolution and are suitable for X-ray structure analysis at high resolution.
