TAT-Liposomes: A Novel Intracellular Drug Carrier

Author(s): V. P. Torchilin, T. S. Levchenko

Journal Name: Current Protein & Peptide Science

Volume 4 , Issue 2 , 2003


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Abstract:

TAT peptide was attached to the surface of plain and PEGylated liposomes. These TAT peptide-modified liposomes have been shown to translocate into a variety of normal and cancer cells if a non-hindered interaction between the cell surface and liposome-attached TAT peptide was made possible. TAT peptide-liposomes translocated into cells remain intact within first few hours as proved by a co-localization of fluorescent markers entrapped inside liposomes and incorporated into the liposomal membrane. After 2 hours liposomes had slowly migrating towards cell nuclei. Liposomes had completely disintegrated with their inner marker released by approximately 9 hours. TAT peptide-liposomes were made slightly cationic by adding up to 10 mol % of a cationic lipid (DOTAP). These slightly cationic liposomes were non-toxic towards cells, formed firm complexes with DNA (plasmid encoding for the formation of the Green Fluorescent Protein), and efficiently transfected a variety of cells. TAT peptideliposomes can be considered as promising carriers for the non-endocytotic intracellular delivery of drugs and DNA.

Keywords: TAT-Liposomes, peptide, PEGylated, liposomes, non-endocytotic

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Article Details

VOLUME: 4
ISSUE: 2
Year: 2003
Page: [133 - 140]
Pages: 8
DOI: 10.2174/1389203033487298
Price: $65

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