Abstract
The methanol extract from Salvia miltiorrhiza Bungee showed a suppressive effect on umu gene expression of the SOS response in Salmonella typhimurium TA1535 / pSK1002 against the mutagen, Trp-P-1, which requires liver metabolizing enzyme. The methanol extract was successively re-extracted with dichloromethane, butanol, and water. Three suppressive compounds were isolated by SiO2 column chromatography from dichloromethane fraction and identified as tanshinone IIA (1), tanshinone I (2) and Cryptotanshinone (3) by GC, GC-MS, 1H- and 13C-NMR spectroscopy. Compounds 1, 2 and 3 inhibited the SOS-inducing activity of Trp-P-1 in the umu test. Gene expression was suppressed by 76%, 96% and 81% at less than 0.18 μmol / mL, 9 respectively. The ID50 (50% inhibition dose) values were 0.03 μmol / mL 0.008 μmol / mL and 0.010 μmol / mol, respectively. These compounds were assayed with activated Trp-P-1. Compound 2 showed suppressive effect of SOS-inducing activity of activated Trp-P-1 but compounds 1 and 3 did not.
Keywords: salvia miltiorrhiza bunge, diterpenoid, tanshinone, sos response, umu test, antimutagen
Letters in Drug Design & Discovery
Title: Suppressive Components in Salvia miltiorrhiza Against Trp-P-1 and Activated Trp-P-1-Induced SOS Response Using Salmonella typhimurium TA1535 / pSK1002 Umu Test
Volume: 1 Issue: 1
Author(s): Yoshiharu Okuno and Mitsuo Miyazawa
Affiliation:
Keywords: salvia miltiorrhiza bunge, diterpenoid, tanshinone, sos response, umu test, antimutagen
Abstract: The methanol extract from Salvia miltiorrhiza Bungee showed a suppressive effect on umu gene expression of the SOS response in Salmonella typhimurium TA1535 / pSK1002 against the mutagen, Trp-P-1, which requires liver metabolizing enzyme. The methanol extract was successively re-extracted with dichloromethane, butanol, and water. Three suppressive compounds were isolated by SiO2 column chromatography from dichloromethane fraction and identified as tanshinone IIA (1), tanshinone I (2) and Cryptotanshinone (3) by GC, GC-MS, 1H- and 13C-NMR spectroscopy. Compounds 1, 2 and 3 inhibited the SOS-inducing activity of Trp-P-1 in the umu test. Gene expression was suppressed by 76%, 96% and 81% at less than 0.18 μmol / mL, 9 respectively. The ID50 (50% inhibition dose) values were 0.03 μmol / mL 0.008 μmol / mL and 0.010 μmol / mol, respectively. These compounds were assayed with activated Trp-P-1. Compound 2 showed suppressive effect of SOS-inducing activity of activated Trp-P-1 but compounds 1 and 3 did not.
Export Options
About this article
Cite this article as:
Okuno Yoshiharu and Miyazawa Mitsuo, Suppressive Components in Salvia miltiorrhiza Against Trp-P-1 and Activated Trp-P-1-Induced SOS Response Using Salmonella typhimurium TA1535 / pSK1002 Umu Test, Letters in Drug Design & Discovery 2004; 1 (1) . https://dx.doi.org/10.2174/1570180043485635
DOI https://dx.doi.org/10.2174/1570180043485635 |
Print ISSN 1570-1808 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-628X |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Stability in Cognitive Function Over 18 Years: Prevalence and Predictors among Older Mexican Americans
Current Alzheimer Research Formulation and Characterization of Rutin Loaded Chitosan-alginate Nanoparticles: Antidiabetic and Cytotoxicity Studies
Current Drug Delivery The Role of Coronary Collaterals in Chronic Total Occlusions
Current Cardiology Reviews Conserved Molecular Players for Axon Guidance and Angiogenesis
Current Protein & Peptide Science Epilepsy and Related Neuropsychiatric Comorbidities: Basic and Clinical Research
Current Neuropharmacology Hypertension in Obesity: Novel Insights
Current Hypertension Reviews Pharmaceutical and Biomedical Analysis of Terpene Constituents in Salvia miltiorrhiza
Current Pharmaceutical Analysis Does Pharmacological Therapy Still Play a Role in Preventing Sudden Death in Surgically Treated Tetralogy of Fallot?
Mini-Reviews in Medicinal Chemistry From Discovery to Clinical Trials: Treatment Strategies for Central Neuropathic Pain after Spinal Cord Injury
Current Pharmaceutical Design Significant Association of CXCL12 rs1746048 with LDL-C Level in Intracranial Aneurysms
Current Neurovascular Research Pathological Roles of Iron in Cardiovascular Disease
Current Drug Targets Anti-platelet Drug-loaded Targeted Technologies for the Effective Treatment of Atherothrombosis
Current Drug Targets Translocator Protein (TSPO) and Neurosteroids: Implications in Psychiatric Disorders
Current Molecular Medicine Pulmonary Hypertension: Clinical Presentation, Diagnosis, Treatment,and Dana Point World Symposium Highlights
Current Respiratory Medicine Reviews <i>In Vivo/Ex Vivo</i> EPR Investigation of the Brain Redox Status and Blood-Brain Barrier Integrity in the 5xFAD Mouse Model of Alzheimer's Disease
Current Alzheimer Research Secondary Hypertension: The Ways of Management
Current Vascular Pharmacology ATP-Adenosine-Glutathione Cross-Linked Hemoglobin as Clinically Useful Oxygen Carrier
Current Drug Discovery Technologies ACAT1 as a Therapeutic Target and its Genetic Relationship with Alzheimer's Disease
Current Alzheimer Research Subject Index To Volume 4
Current Vascular Pharmacology Ideational Fluency in Patients with Rheumatoid Arthritis
Current Rheumatology Reviews