Modifications in the Purification Protocol of Celosia Cristata Antiviral Proteins Lead to Protein that Can Be N-Terminally Sequenced

Author(s): Ashraf Gholizadeh, H. C. Kapoor.

Journal Name: Protein & Peptide Letters

Volume 11 , Issue 6 , 2004

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Abstract:

Plants antiviral proteins are being used as anticancer agents and inhibit other viral diseases in humans. We modified the purification protocol of the two N-terminally blocked antiviral glycoproteins, CCP-25 and CCP-27, purified from the leaves of Celosia cristata. This not only gave rise to single pure samples with few steps of purification but also resulted in N-terminally free proteins. The extra purity of the samples was analyzed by reverse phase HPLC. Deglycosylation studies of CCP-25 with PNGase F enzyme revealed that its asparagine or asparagine-linked glycon contents are negligible. Partial N-terminal sequence of the CCP-25 showed the sequence (ANDIS), which seems to be conserved among plant antiviral proteins.

Keywords: antiviral, proteins, glycoproteins, celosia, purification

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Article Details

VOLUME: 11
ISSUE: 6
Year: 2004
Page: [555 - 561]
Pages: 7
DOI: 10.2174/0929866043406210
Price: $65

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