Preferential Codons Enhancing the Expression Level of Human Beta-Defensin-2 in Recombinant Escherichia Coli

Author(s): Li Peng, Zhinan Xu, Xiangming Fang, Fang Wang, Sheng Yang, Peilin Cen

Journal Name: Protein & Peptide Letters

Volume 11 , Issue 4 , 2004

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Abstract:

Human β-defensin-2 (hBD2) is a small antimicrobial peptide with potential as a therapeutic agent. The effect of codon usage on the expression of hBD2 in Escherichia coli was studied. Two coding sequences encoding the same hBD2 precursor were both expressed as fusion protein with thioredoxin in E. coli BL21 (DE3). One is the wild-type human cDNA and the other is a gene synthesized by a PCR-based method in which rare codons were altered to those frequently used in E. coli. The expression level of recombinant hBD2 was over 50% of the total cellular protein when the synthetic gene with preferential codons was employed which was a 9-fold enhancement over the wild-type cDNA. The result shows the codon bias of the host was a major barrier in high-level expression of recombinant hBD2 and suggests a similar approach may be used in the expression of other defensins in E. coli.

Keywords: antimicrobial peptide, codon usage, human defensin-2, gene synthesis

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Article Details

VOLUME: 11
ISSUE: 4
Year: 2004
Page: [339 - 344]
Pages: 6
DOI: 10.2174/0929866043406760
Price: $65

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