Affinity Ligands for Industrial Protein Purification

Author(s): Martin Linhult, Susanne Gulich, Sophia Hober

Journal Name: Protein & Peptide Letters

Volume 12 , Issue 4 , 2005

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Significant efforts are put into the design of large-scale purification processes of proteins due to great demands regarding cost efficiency and safety. In order to design an effective purification scheme the unit operations need to be reduced to a minimum. In this review we are discussing proteinaceous ligands as well as small synthetic mimics for use in affinity chromatography for large-scale applications. Different advantages as well as drawbacks of the two approaches are outlined.

Keywords: affinity chromatography, cleaning-in-place (cip), deamidation, protein a, protein g, protein engineering, stabilization

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Article Details

Year: 2005
Page: [305 - 310]
Pages: 6
DOI: 10.2174/0929866053765662
Price: $65

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