Title: Mild and Facile Deprotection for the Synthesis of Oligodeoxynucleotide Incorporating a 6-O-Ethyl-deoxyguanosine
VOLUME: 2 ISSUE: 2
Author(s):Kohji Seio, Tomoe Negishi, Kazuo Negishi and Mitsuo Sekine
Affiliation:Department of Life Science, Tokyo Institute of Technology, Nagatsuta, Midoriku, Yokohama, 226-8501, Japan.
Keywords:6-o-ethylguanine, degenerated base, mutation, phenoxyacetyl group, depurination, oligodeoxynucleotide synthesis
Abstract: The phenoxyacetyl (Pac) group proved to be useful for the synthesis of oligodeoxyribonucleotides incorporating a 6-O-ethylguanine. Since the Pac group could be removed by mild treatment with ammonia, formation of a 2, 4-diaminopurine derivative was avoided. Moreover, the use of the Pac group resulted in significant suppression of the depurination of 6-O-ethyldeoxyguanosine.
