Abstract
This review briefly covers recent literature of research on the phosphoryl transfer mechanism of PKA. Combining experimental and theoretical calculation results on enzymes with experimentally observed biomimic activities of phosphoryl amino acids and a small molecular model of catalytic core in PKA, a novel mechanism was proposed. The cooperative participation roles of both Asp166 and Lys168 via a penta-coodinate phosphoric intermediate was elucidated to conciliate the current different views of the phosphoryl transfer mechanism of PKA. Since many ATP-binding enzymes may share a similar phosphoryl transfer mechanism, this proposed mechanism might also apply to the mechanism of these enzymes, e.g., molecular motor and phosphatase among others.
Keywords: camp dependent protein kinase (pka), phosphoryl, amino acid, penta-coodinate, phosphoric intermediate, hexacoodinate phosphoric intermediate, phosphoryl transfer mechanism, lysine participation
Current Protein & Peptide Science
Title: Analysis of the Phosphoryl Transfer Mechanism of c-AMP dependent Protein Kinase (PKA) by Penta-Coodinate Phosphoric Transition State Theory
Volume: 6 Issue: 5
Author(s): Feng Ni, Wu Li, Yan-Mei Li and Yu-Fen Zhao
Affiliation:
Keywords: camp dependent protein kinase (pka), phosphoryl, amino acid, penta-coodinate, phosphoric intermediate, hexacoodinate phosphoric intermediate, phosphoryl transfer mechanism, lysine participation
Abstract: This review briefly covers recent literature of research on the phosphoryl transfer mechanism of PKA. Combining experimental and theoretical calculation results on enzymes with experimentally observed biomimic activities of phosphoryl amino acids and a small molecular model of catalytic core in PKA, a novel mechanism was proposed. The cooperative participation roles of both Asp166 and Lys168 via a penta-coodinate phosphoric intermediate was elucidated to conciliate the current different views of the phosphoryl transfer mechanism of PKA. Since many ATP-binding enzymes may share a similar phosphoryl transfer mechanism, this proposed mechanism might also apply to the mechanism of these enzymes, e.g., molecular motor and phosphatase among others.
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Cite this article as:
Ni Feng, Li Wu, Li Yan-Mei and Zhao Yu-Fen, Analysis of the Phosphoryl Transfer Mechanism of c-AMP dependent Protein Kinase (PKA) by Penta-Coodinate Phosphoric Transition State Theory, Current Protein & Peptide Science 2005; 6 (5) . https://dx.doi.org/10.2174/138920305774329296
DOI https://dx.doi.org/10.2174/138920305774329296 |
Print ISSN 1389-2037 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5550 |
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