Crystallization and Preliminary X-Ray Diffraction Studies of Two Myotoxic Lys49-Phospholipases A2 Complexed with α-Tocopherol

Author(s): Juliana I. dos Santos, Agnes A.S. Takeda, Daniela P. Marchi-Salvador, Andreimar M. Soares, Marcos R.M. Fontes

Journal Name: Protein & Peptide Letters

Volume 12 , Issue 8 , 2005

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Abstract:

BnSP-7 and BnSP-6, two Lys49-phospholipase A2 isolated from Bothrops neuwiedi pauloensis snake venom, were co-crystallized with α-tocopherol and X-ray diffraction data were collected for both complexes (2.2 and 2.6 Å). A new "alternative" quaternary conformation for these two complexes compared with all other dimeric Lys49-PLA2 has been observed.

Keywords: crystallization, x-ray crystallography, lys-phospholipase a, bothrops neuwiedi venom, myotoxity, tocopherol

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Article Details

VOLUME: 12
ISSUE: 8
Year: 2005
Page: [819 - 822]
Pages: 4
DOI: 10.2174/0929866054864283
Price: $65

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