Incorporation of Selenocysteine into Proteins Using Peptide Ligation

Author(s): Robert J. Hondal

Journal Name: Protein & Peptide Letters

Volume 12 , Issue 8 , 2005


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Abstract:

Expressed protein ligation has become a frequently used technique to insert non-standard amino acids into proteins. The technique has been adapted to insert selenocysteine residues in place of cysteine residue in proteins, taking advantage of the similarity in the chemistries of sulfur and selenium. This replacement can confer unique structural and catalytic properties to enzymes and proteins. The development of this technique also allows for naturally occurring selenoproteins to be produced semisynthetically.

Keywords: selenocysteine, expressed protein ligation, thioredoxin reductase, semisynthetic

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Article Details

VOLUME: 12
ISSUE: 8
Year: 2005
Page: [757 - 764]
Pages: 8
DOI: 10.2174/0929866054864319
Price: $65

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