Expression of a Lipid Transfer Protein in Escherichia coli and Its Phosphorylation by a Membrane-Bound Calcium-Dependent Protein Kinase

Author(s): M.L. Martin, E. Espinosa Vidal, L. de la Canal

Journal Name: Protein & Peptide Letters

Volume 14 , Issue 8 , 2007


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Abstract:

Ha-AP10 is a basic antifungal peptide from sunflower seeds (Helianthus annuus antifungal peptide of 10 kDa) belonging to the family of plant lipid transfer proteins. We report here its expression in E. coli [Glutathione S-transferase (GST) system] and its phosphorylation by endogenous membrane-bound calcium-dependent protein kinases.

Keywords: Calcium-dependent protein phosphorylation, GST expression system, Helianthus annuus, lipid transfer protein

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Article Details

VOLUME: 14
ISSUE: 8
Year: 2007
Page: [793 - 799]
Pages: 7
DOI: 10.2174/092986607781483697
Price: $65

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