Expression and Purification of Uniformly 15N-Labeled Amyloid β Peptide 1-40 in Escherichia coli

Author(s): M. Nagata-Uchiyama, M. Yaguchi, Y. Hirano, T. Ueda

Journal Name: Protein & Peptide Letters

Volume 14 , Issue 8 , 2007


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Abstract:

For biophysical studies using heteronuclear NMR analysis of amyloid beta peptide, construction of an efficient and high yield expression system of uniformly isotopic labeled amyloid beta peptide is desirable. Here we succeeded in obtaining 15N-labeled amyloid beta 1-40 expressed by attachment to hen egg white lysozyme as a fusion protein.

Keywords: Alzheimer's disiease, amyloid β 1-40, amyloid fibrils, recombinant protein, Escherichia coli, NMR

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Article Details

VOLUME: 14
ISSUE: 8
Year: 2007
Published on: 01 March, 2012
Page: [788 - 792]
Pages: 5
DOI: 10.2174/092986607781483741
Price: $65

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