Cautionary Tail: The Presence of an N-Terminal Tag on Dynein Light-Chain Roadblock/LC7 Affects Its Interaction with a Functional Partner

Author(s): Jikui Song, John L. Markley, Jikui Song, John L. Markley

Journal Name: Protein & Peptide Letters

Volume 14 , Issue 3 , 2007

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As part of structural investigations of components of the molecular motor, dynein, we prepared the light chain, Robl1_mouse, with and without an N-terminal His-tag. We found that the His-tag introduced a spurious binding site for a second protein, IC74. We propose a molecular mechanism for functional interference by the His-tag.

Keywords: Affinity tag, docking, dynein, NMR spectroscopy, protein dynamics, protein-protein interactions

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Article Details

Year: 2007
Page: [265 - 268]
Pages: 4
DOI: 10.2174/092986607780090801

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