The fast-twitch SERCA1 isoform of the sarcoplasmic reticulum Ca2+-ATPase was purified to homogeneity and conjugated to peroxidase. The SERCA1 probe showed high affinity binding to the immobilized monomeric enzyme, but not crosslinker-stabilized oligomers. This suggests a preferential complex formation via homo-dimerization, rather than interactions with established oligomeric structures.
Keywords: Blot overlay, Ca2+-ATPase, Ca2+-uptake, oligomerisation, sarcoplasmic reticulum, SERCA
Rights & PermissionsPrintExport