Oligomerisation of Sarcoplasmic Reticulum Ca2+-ATPase Monomers from Skeletal Muscle

Author(s): Daniela Schreiber, Kay Ohlendieck

Journal Name: Protein & Peptide Letters

Volume 14 , Issue 3 , 2007

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The fast-twitch SERCA1 isoform of the sarcoplasmic reticulum Ca2+-ATPase was purified to homogeneity and conjugated to peroxidase. The SERCA1 probe showed high affinity binding to the immobilized monomeric enzyme, but not crosslinker-stabilized oligomers. This suggests a preferential complex formation via homo-dimerization, rather than interactions with established oligomeric structures.

Keywords: Blot overlay, Ca2+-ATPase, Ca2+-uptake, oligomerisation, sarcoplasmic reticulum, SERCA

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Article Details

Year: 2007
Page: [219 - 226]
Pages: 8
DOI: 10.2174/092986607780090838
Price: $65

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