Large Scale Preparation of the Mammalian High Mobility Group Protein A2 for Biophysical Studies

Author(s): Tengjiao Cui, Suzanne Joynt, Victor Morillo, Maria Baez, Zhichun Hua, Xiaotang Wang, Fenfei Leng

Journal Name: Protein & Peptide Letters

Volume 14 , Issue 1 , 2007


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Abstract:

Due to asymmetrical charge distribution of the mammalian high mobility group protein A2 (HMGA2), which makes HMGA2 bind to both cation- and anion-exchange columns, we developed a rapid procedure for purifying HMGA2 in the milligram range. This purification procedure greatly facilitated biophysical studies, which require large amounts of the protein.

Keywords: The mammalian high mobility group protein A2 (HMGA2), DNA binding protein, isothermal titration calorimetry, differential scanning calorimetry, nuclear magnetic resonance (NMR) Spectroscopy, intrinsically unstructured protein

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Article Details

VOLUME: 14
ISSUE: 1
Year: 2007
Page: [87 - 91]
Pages: 5
DOI: 10.2174/092986607779117281

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