Abstract
Advances in novel tumor-associated antigen (TAA) screening strategy have accelerated the identification and characterization of biomarkers and potential target molecules for tumor subtyping, diagnosis and therapeutics, which may facilitate early detection and diagnosis of the diseases individually and enhance treatment approaches for cancer. Over the past decades, a plethora of non-invasive methodologies dedicated to identify novel target molecules have been primarily focusing on the discovery of human tumor antigens recognized by the autologous antibody repertoire or cytotoxic T lymphocytes, among which serological analysis of recombinant cDNA expression libraries (SEREX) technology is chronologically first established and is of outstanding sensitivity and antigen coverage. This approach involves immunoscreening cDNA libraries extracted from fresh tumor tissues with sera from cancer patients to identify gene products recognized by IgG antibody. SEREX-defined clones can be directly sequenced and their expression profiles can be readily determined, allowing for immediate structural definition of the antigenic target and subsequent identification of TAAs and their cognate autoantibodies. This review is not only devoted to outline the SEREX technology and its advantages, drawbacks and recent modifications currently available for discovering provocative tumor antigens, but also to translate these SEREX-defined peptides into valuable cancer-specific signatures that would aid in the development of diagnostics, prognostics and therapeutics for cancer patients.
Keywords: High throughput, SEREX, tumor antigens, biomarkers, target molecules, detection, diseases, recombinant cDNA, IgG antibody, clones, immune system, malignant cells, proteins, transformed cells, ELISAs, antigens, epitopes, T-cells, serum, expressed sequence tag
Combinatorial Chemistry & High Throughput Screening
Title: Mapping the High Throughput SEREX Technology Screening for Novel Tumor Antigens
Volume: 15 Issue: 3
Author(s): Shengtao Zhou, Tao Yi, Boya Zhang, Fuqiang Huang, Huiqiong Huang, Jing Tang and Xia Zhao
Affiliation:
Keywords: High throughput, SEREX, tumor antigens, biomarkers, target molecules, detection, diseases, recombinant cDNA, IgG antibody, clones, immune system, malignant cells, proteins, transformed cells, ELISAs, antigens, epitopes, T-cells, serum, expressed sequence tag
Abstract: Advances in novel tumor-associated antigen (TAA) screening strategy have accelerated the identification and characterization of biomarkers and potential target molecules for tumor subtyping, diagnosis and therapeutics, which may facilitate early detection and diagnosis of the diseases individually and enhance treatment approaches for cancer. Over the past decades, a plethora of non-invasive methodologies dedicated to identify novel target molecules have been primarily focusing on the discovery of human tumor antigens recognized by the autologous antibody repertoire or cytotoxic T lymphocytes, among which serological analysis of recombinant cDNA expression libraries (SEREX) technology is chronologically first established and is of outstanding sensitivity and antigen coverage. This approach involves immunoscreening cDNA libraries extracted from fresh tumor tissues with sera from cancer patients to identify gene products recognized by IgG antibody. SEREX-defined clones can be directly sequenced and their expression profiles can be readily determined, allowing for immediate structural definition of the antigenic target and subsequent identification of TAAs and their cognate autoantibodies. This review is not only devoted to outline the SEREX technology and its advantages, drawbacks and recent modifications currently available for discovering provocative tumor antigens, but also to translate these SEREX-defined peptides into valuable cancer-specific signatures that would aid in the development of diagnostics, prognostics and therapeutics for cancer patients.
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Cite this article as:
Zhou Shengtao, Yi Tao, Zhang Boya, Huang Fuqiang, Huang Huiqiong, Tang Jing and Zhao Xia, Mapping the High Throughput SEREX Technology Screening for Novel Tumor Antigens, Combinatorial Chemistry & High Throughput Screening 2012; 15 (3) . https://dx.doi.org/10.2174/138620712799218572
DOI https://dx.doi.org/10.2174/138620712799218572 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
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