Abstract
Angiotensin-I converting enzyme (ACE) has an important function in blood pressure regulation. ACEinhibitory peptides can lower blood pressure by inhibiting ACE activity. In this investigation, water-soluble proteins were extracted from yeast (Saccharomyces cerevisiae) and hydrolyzed by yeast protein extraction enzyme to isolate ACEinhibitory peptides. Peptides with ACE-inhibitory activity were further separated and purified by ultrafiltration and fast protein liquid chromatography (FPLC). A hexapeptide, Thr-Pro-Thr-Gln-Gln-Ser, with a calculated molecular weight of 660Da, was purified and identified by MALDI-TOF-MS. The hexapeptide showed remarkable ACE-inhibitory activity, with an IC50 of 73.25 μg/mL. The level of ACE-inhibitory activity of the hexapeptide indicated that it is a good candidate for development of a hypotension drug or functional food.
Keywords: Angiotensin-I converting enzyme, Bioactive peptide, Inhibitory peptide, Yeast protein, Fast protein liquid chromatography (FPLC), High performance liquid chromatography, Mass spectrometry, MALDI-TOF-MS, Ultrafiltration, Water-soluble proteins
Current Analytical Chemistry
Title: Isolation and Identification of an Angiotensin-I Converting Enzyme Inhibitory Peptide from Yeast (Saccharomyces cerevisiae)
Volume: 8 Issue: 1
Author(s): He Ni, Lin Li, Sha-Sha Guo, Hai-Hang Li, Rui Jiang and Song-Qing Hu
Affiliation:
Keywords: Angiotensin-I converting enzyme, Bioactive peptide, Inhibitory peptide, Yeast protein, Fast protein liquid chromatography (FPLC), High performance liquid chromatography, Mass spectrometry, MALDI-TOF-MS, Ultrafiltration, Water-soluble proteins
Abstract: Angiotensin-I converting enzyme (ACE) has an important function in blood pressure regulation. ACEinhibitory peptides can lower blood pressure by inhibiting ACE activity. In this investigation, water-soluble proteins were extracted from yeast (Saccharomyces cerevisiae) and hydrolyzed by yeast protein extraction enzyme to isolate ACEinhibitory peptides. Peptides with ACE-inhibitory activity were further separated and purified by ultrafiltration and fast protein liquid chromatography (FPLC). A hexapeptide, Thr-Pro-Thr-Gln-Gln-Ser, with a calculated molecular weight of 660Da, was purified and identified by MALDI-TOF-MS. The hexapeptide showed remarkable ACE-inhibitory activity, with an IC50 of 73.25 μg/mL. The level of ACE-inhibitory activity of the hexapeptide indicated that it is a good candidate for development of a hypotension drug or functional food.
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Ni He, Li Lin, Guo Sha-Sha, Li Hai-Hang, Jiang Rui and Hu Song-Qing, Isolation and Identification of an Angiotensin-I Converting Enzyme Inhibitory Peptide from Yeast (Saccharomyces cerevisiae), Current Analytical Chemistry 2012; 8 (1) . https://dx.doi.org/10.2174/157341112798472224
DOI https://dx.doi.org/10.2174/157341112798472224 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
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