A kind of univariate calibration, net analyte signal combined with standard addition (NAS-SAM) is presented for determination of Guaifenesin, Pseudoephedrine and Chlorpheniramine in the presence of each other by spectrophotometry. The method has both the advantages of the standard addition method and net analyte signal which enables the extraction of information concerning a certain analyte from spectra of multi-component mixtures. The use of full spectrum to calculate the net analyte signal vector and also no requirement of calibration and prediction steps are the most important advantages of NAS-SAM. The simultaneous determination of Guaifenesin, Pseudoephedrine and Chlorpheniramine was performed in Britton-Robinson buffer (pH 9). Three pharmaceutical compounds were determined in concentration range of 0.5-100 μmol L-1 In this work, the method of NAS-SAM has been used for the determination of guaifenesin, pseudoephedrine and chlorpheniramine in some ternary mixtures and cough syrups with satisfactory results.
Keywords: Chemometrics, Chlorpheniramine, Correlation, Full Spectrum, Guaifenesin, NAS-SAM, Orthogonalization, Overlapping spectra, Pseudoephedrine, Syrups
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