Abstract
Food allergy has become a serious human health concern. With more than 160 kinds of food allergens have been identified to date, allergen detection has become challenging. Most current allergen detection methods are capable of detecting only a few allergens at a time. The objective of this work was to develop a sensitive method for the detection of fish parvalbumin in food matrices employing a 3D aldehyde protein chip, which has the capacity to detect several allergens simultaneously. Upon testing, the protein chip was found to be highly specific in allergen detection but was found to cross-react to some extent with the allergens from some closely related fish species. The allergen recoveries ranged from 72.1% to 95.2%. The inter- and intra- assay coefficients of variation were < 18% and < 12% respectively. The LOD (limit of detection) and LOQ (limit of quantitation) of the protein chip of 0.048 and 0.087 mg parvalbumin/kg food, respectively, was sensitive enough given the threshold limit for the fish allergen. The newly developed assay seems reliable enough in detecting fish parvalbumin from various foods, food products and thus could prove useful in detecting and minimizing the instances of fish allergy.
Keywords: Allergen, Protein chip, Fish, Parvalbumin, Detection, Food allergy, 3D aldehyde protein chip, Limit of quantitation, Limit of detection, Threshold limit
Current Analytical Chemistry
Title: Development of an Optimized Protein Chip for the Detection of Fish Parvalbumin Allergen
Volume: 7 Issue: 4
Author(s): Zhenxing Li, Yiqun Zhang, Ravindra Pawar, Guoying Wang and Hong Lin
Affiliation:
Keywords: Allergen, Protein chip, Fish, Parvalbumin, Detection, Food allergy, 3D aldehyde protein chip, Limit of quantitation, Limit of detection, Threshold limit
Abstract: Food allergy has become a serious human health concern. With more than 160 kinds of food allergens have been identified to date, allergen detection has become challenging. Most current allergen detection methods are capable of detecting only a few allergens at a time. The objective of this work was to develop a sensitive method for the detection of fish parvalbumin in food matrices employing a 3D aldehyde protein chip, which has the capacity to detect several allergens simultaneously. Upon testing, the protein chip was found to be highly specific in allergen detection but was found to cross-react to some extent with the allergens from some closely related fish species. The allergen recoveries ranged from 72.1% to 95.2%. The inter- and intra- assay coefficients of variation were < 18% and < 12% respectively. The LOD (limit of detection) and LOQ (limit of quantitation) of the protein chip of 0.048 and 0.087 mg parvalbumin/kg food, respectively, was sensitive enough given the threshold limit for the fish allergen. The newly developed assay seems reliable enough in detecting fish parvalbumin from various foods, food products and thus could prove useful in detecting and minimizing the instances of fish allergy.
Export Options
About this article
Cite this article as:
Li Zhenxing, Zhang Yiqun, Pawar Ravindra, Wang Guoying and Lin Hong, Development of an Optimized Protein Chip for the Detection of Fish Parvalbumin Allergen, Current Analytical Chemistry 2011; 7 (4) . https://dx.doi.org/10.2174/157341111797183100
DOI https://dx.doi.org/10.2174/157341111797183100 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Analytical methods for environmental and food analysis
In recent years, the study of the chemical composition of food and the environment depends on analytical methods in order to acquire information about the chemical composition, processing, quality control and contamination of soil, water, air, plants and foods, aspects very important for food safety and environmental quality control. There ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
Bioanalytical and Biosensor Technologies for Target Molecules
Bioanalytical and biosensor technologies constitute the core of detecting and sensing diverse molecules within living organisms, encompassing proteins, DNA, small molecular metabolites, and beyond. These molecules assume pivotal roles in biological processes, rendering their analysis and sensing indispensable for comprehending biological phenomena and unraveling disease mechanisms. In the current landscape ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Genoprotective Effect of New Triazine Derivatives in Endosulfan Mediated Toxicity, an In vivo and In vitro Study
Letters in Drug Design & Discovery Small Yet Mighty – MicroRNAs in Plant-Microbe Interactions
MicroRNA Current Scenario and Future Prospect in the Management of COVID-19
Current Medicinal Chemistry Targeting Mutant KRAS for Anticancer Therapy
Current Topics in Medicinal Chemistry Recent Patents in Oncolytic Virotherapy
Recent Patents on Biotechnology Pharmacological Agents in the Treatment of Venous Disease: An Update of the Available Evidence
Current Vascular Pharmacology Biophysical and Structural Characterization of the Recombinant Human eIF3L
Protein & Peptide Letters Anti-TNFs for Postoperative Recurrence in Crohns Disease: The Ifs and Hows
Current Drug Targets Cancer-Associated Carbonic Anhydrases and Their Inhibition
Current Pharmaceutical Design Protein-Protein Interactions: Recent Progress in the Development of Selective PDZ Inhibitors
Current Chemical Biology Bacterial Adaptation and Infection
Inflammation & Allergy - Drug Targets (Discontinued) The Assessment of Anxiety and Depression and its Associated Factors in SLE
Current Rheumatology Reviews The CX3C-Chemokine Fractalkine in Kidney Diseases
Mini-Reviews in Medicinal Chemistry Inhibition of Sumoylation Alleviates Oxidative Stress-induced Retinal Pigment Epithelial Cell Senescence and Represses Proinflammatory Gene Expression
Current Molecular Medicine Relevance of Aβ 1-42 Intrahippocampal Injection as An Animal Model of Inflamed Alzheimers Disease Brain
Current Alzheimer Research Drug Delivery to the Inner Ear: Strategies and Their Therapeutic Implications for Sensorineural Hearing Loss
Current Drug Delivery Chelating Agents for Metal Intoxication
Current Medicinal Chemistry Magnitude and Clinical Profile of Juvenile Idiopathic Arthritis in a Medical College Hospital of a Socio-economically Disadvantaged Country
Current Rheumatology Reviews Toll-Like Receptors in the Cycling Female Reproductive Tract and During Pregnancy
Current Women`s Health Reviews Stimulation of DDX3 Expression by Ginsenoside Rg3 through the Akt/p53 Pathway Activates the Innate Immune Response via TBK1/IKKε/IRF3 Signalling
Current Medicinal Chemistry