Development of a Novel Reporter Gene Vector for Cell Based Angiogenic Studies

Author(s): Srinivasan Kokatam, Deepshikha Pandey, Ramkrishna Gupta, Rathu Krishna, Rajeev Soni

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 14 , Issue 8 , 2011

Become EABM
Become Reviewer
Call for Editor


Angiogenesis is a promising area of research that targets key therapeutic areas like cancer; wound healing, inflammatory diseases, etc. There is an increasing demand for screening of potential angiogenic and anti-angiogenic agents using sensitive, robust cell-based assays. We have developed a reporter vector containing cis-acting elements that respond to growth factors/angiogenic ligands for use in a cell-based luciferase reporter assay. We performed transient transfection of our reporter gene vector in MCF-7 cells to establish its application for screening of potential pro/antiangiogenic agents. Reporter gene transactivation studies with different concentrations of fetal bovine serum clearly indicated that the vector is functionally responsive to the angiogenic signals mediated by serum growth factors. We also used endostatin to inhibit transactivation and prove responsiveness to the anti-angiogenic agent. This vector is a promising tool for studying angiogenesis using cell-based reporter gene assays.

Keywords: Angiogenesis, endostatin, MCF-7, screening, serum, transfection, Cell Based, Reporter Gene Vector, inflammatory diseases, transactivation, extracellular matrix degradation, in vitro

Rights & PermissionsPrintExport Cite as

Article Details

Year: 2011
Page: [703 - 709]
Pages: 7
DOI: 10.2174/138620711796504361
Price: $65

Article Metrics

PDF: 6