Iron has been quantified in pharmaceutical preparations by developing red spots pursuant to interaction of Fe(II) ions in the sample with 1, 10-phenanthroline on TLC plate. Soon after, TLC was scanned on a flatbed scanner and the image was transferred to the computer. Color intensity of the spot was computationally quantified with the help of native software developed for this purpose. The conditions were optimized and the results were compared with a reference method.
Keywords: Computational quantification, Densitometry, Image scanning, Iron determination, Spot test, TLC, Spectrophotometry, electromagnetic radiations, precipitation, serum, pH, mobile phase, RGB, spectrophotometer, Stock solution, micropipette, density value, Absorbance, Calibration line, concentrations, iron
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