Controlled Somatic and Germline Copy Number Variation in the Mouse Model

Author(s): Yann Herault, Arnaud Duchon, Damien Marechal, Matthieu Raveau, Patricia L. Pereira, Emilie Dalloneau, Veronique Brault

Journal Name: Current Genomics

Volume 11 , Issue 6 , 2010

Become EABM
Become Reviewer

Abstract:

Changes in the number of chromosomes, but also variations in the copy number of chromosomal regions have been described in various pathological conditions, such as cancer and aneuploidy, but also in normal physiological condition. Our classical view of DNA replication and mitotic preservation of the chromosomal integrity is now challenged as new technologies allow us to observe such mosaic somatic changes in copy number affecting regions of chromosomes with various sizes. In order to go further in the understanding of copy number influence in normal condition we could take advantage of the novel strategy called Targeted Asymmetric Sister Chromatin Event of Recombination (TASCER) to induce recombination during the G2 phase so that we can generate deletions and duplications of regions of interest prior to mitosis. Using this approach in the mouse we could address the effects of copy number variation and segmental aneuploidy in daughter cells and allow us to explore somatic mosaics for large region of interest in the mouse.

Keywords: Chromosomal engineering, Cre/loxP, mosaic genetics, duplication, deletion

Rights & PermissionsPrintExport Cite as

Article Details

VOLUME: 11
ISSUE: 6
Year: 2010
Page: [470 - 480]
Pages: 11
DOI: 10.2174/138920210793176038
Price: $65

Article Metrics

PDF: 1