Development and Application of a Highly Selective Biomimetic Sensor for Detection of Captopril, an Important Ally in Hypertension Control

Author(s): Ademar Wong, Marcos R.V. Lanza, Maria D.P.T. Sotomayor

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 13 , Issue 8 , 2010

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A biomimetic sensor is proposed as a promising new analytical method for determination of captopril in different classes of samples. The sensor was prepared by modifying a carbon paste electrode with iron (II) phthalocyanine bis(pyridine) [FePc(dipy)] complex. Amperometric measurements in a batch analytical mode were first carried out in order to optimize the sensor response. An applied potential lower than 0.2 V vs Ag|AgCl in 0.1 mol L-1 of TRIS buffer at pH 8.0 provided the best response, with a linear range of 2.5 x 10-5 to 1.7 x 10-4 mol L-1. A detailed investigation of the selectivity of the sensor, employing seventeen other drugs, was also performed. Recovery studies were carried out using biological and environment samples in order to evaluate the sensors potential for use with these sample classes. Finally, the performance of the biomimetic sensor was optimized in a flow injection (FIA) system using a wall jet electrochemical cell. Under optimized flow conditions, a broad linear response range, from 5.0 x 10-4 to 2.5 x 10-2 mol L-1, was obtained for captopril, with a sensitivity of 210 ± 1 μA L mol-1.

Keywords: Iron phthalocyanine, biomimetic sensor, captopril, FIA analysis

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Article Details

Year: 2010
Published on: 01 March, 2012
Page: [666 - 674]
Pages: 9
DOI: 10.2174/138620710791920392
Price: $65

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