Engineering of Enzymes for Selective Catalysis

Author(s): Nikhil U. Nair, Carl A. Denard, Huimin Zhao

Journal Name: Current Organic Chemistry

Volume 14 , Issue 17 , 2010

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Naturally occurring enzymes are truly remarkable catalysts. However, many are not suitable for practical synthetic chemistry because of poor substrate or product selectivity. This review highlights recent advances in engineering enzymes for selective catalysis. Selected topics include altering substrate specificity, altering substrate and product selectivity, engineering enzymes that catalyze carboncarbon bond formation or carbon-oxygen bond cleavage and formation, and engineering multi-function enzymes such as polyketide synthases.

Keywords: Directed evolution, enzyme engineering, biocatalysis, selectivity, Enzymes, biocatalysts, epPCR, DNA shuffling, StEP, enantioselectivity, regioselectivity, polyketide synthases, Cytochrome P450 monooxygenases, Bacillus megaterium, FAD, (FMN), NADPH, NADH, UTP, UGPase, (UNGPase), creatine kinase, glycocyamine kinases, E.coli, B.megaterium, C3-C8, MAO-N, Pseudomonas aeruginosa, FDH, Candida boidinii, (PTDH), Pseudomonas stutzeri, TSSM, XR, FDA, HPLC, CASTing, L-methionine, hydantoinase, L-enantiomer, GeneReassembly, cytochrome P450 2B1, (C-C) bond formation, DERA, Lipitor, (NAL), BFD, Pseudomonas putida, Glycosidases, Polyketides, Type I PKSs, Type II PKSs, Type III PKSs, (TAL), FACS

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Article Details

Year: 2010
Page: [1870 - 1882]
Pages: 13
DOI: 10.2174/138527210792927537
Price: $58

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PDF: 13