A Sensitive Voltammetric Sensor for Detecting Betamethasone in Biological Fluids

Author(s): Rajendra N. Goyal, Sunita Bishnoi, Anoop Raj S. Rana

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 13 , Issue 7 , 2010


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Abstract:

A rapid and sensitive voltammetric sensor based on reduction of betamethasone has been developed using single wall carbon nanotube modified edge plane pyrolytic graphite electrode (SWNT/EPPGE). The reduction of betamethasone occurred in a well-defined, pH dependent peak. Linear calibration curve was obtained in the range 1 to 25 nM in 1.0 M phosphate buffer solution (PBS) of pH 7.2 with the limit of detection (3/slope) as 0.50 nM. The analytical utility of the developed method has been demonstarted by sensing the drug in human body fluids and for the determination of betamethasone content in several commercially available pharmaceutical preparations. Interfering effect of some common metabolites including ascorbic acid, uric acid, albumin and hypoxanthine has also been evaluated. A comparison of the observed results of proposed method with HPLC clearly indicates that the results of both methods are essentially similar.

Keywords: Betamethasone, voltammetry, carbon nanotubes, doping, biological fluids

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Article Details

VOLUME: 13
ISSUE: 7
Year: 2010
Page: [610 - 618]
Pages: 9
DOI: 10.2174/1386207311004070610
Price: $65

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