A sensitive aptamer based electrochemical biosensor to detect human immunoglobulin E (IgE) is presented in this study. 5 Biotin labeled 45 mer DNA aptamer sequence was immobilized onto streptavidin coated graphite surfaces. Interaction between human IgE and DNA aptamer was monitored by Electrochemical Impedance Spectrometry (EIS) in a 0.48nM detection limit of IgE. EIS analyses are based on electron transfer resistance (Rct) in the presence of 5mM [Fe(CN)6]3-/4-.
Keywords: Aptamer, IgE, biosensor, electrochemical impedance spectrometry (EIS)
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