Development of a Label Free IGE Sensitive Aptasensor Based on Electrochemical Impedance Spectrometry

Author(s): Pinar Kara, Buket Meric, Mehmet Ozsoz

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 13 , Issue 7 , 2010


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Abstract:

A sensitive aptamer based electrochemical biosensor to detect human immunoglobulin E (IgE) is presented in this study. 5 Biotin labeled 45 mer DNA aptamer sequence was immobilized onto streptavidin coated graphite surfaces. Interaction between human IgE and DNA aptamer was monitored by Electrochemical Impedance Spectrometry (EIS) in a 0.48nM detection limit of IgE. EIS analyses are based on electron transfer resistance (Rct) in the presence of 5mM [Fe(CN)6]3-/4-.

Keywords: Aptamer, IgE, biosensor, electrochemical impedance spectrometry (EIS)

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Article Details

VOLUME: 13
ISSUE: 7
Year: 2010
Page: [578 - 581]
Pages: 4
DOI: 10.2174/1386207311004070578
Price: $65

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