Nano scale chiral separation is essential and has become the demand of current century in drugs development, proteomic, genomic and environmental sciences. Some drugs remain in our body tissues for several weeks at nano scale, which are out of the domain of detection by conventional analytical methods. Similarly, the analyses and detection of xenobiotics; present at nano or low levels; are essential for our health. The present review describes the state-of-art of nano scale chiral separations by nano liquid chromatography (NLC) and nano capillary electrophoresis (NCE). The optimization strategies of chiral separations have been also discussed. Furthermore, efforts were made to discuss the mechanisms of chiral separations in NLC and NCE.