Title: Simultaneous Determination of L- and D-T4 Using a Sequential Injection Analysis/Sensors System
VOLUME: 13 ISSUE: 6
Author(s):Raluca-Ioana Stefan van Staden, Jacobus Frederick van Staden, Hassan Y. Aboul-Enein and Ionel Balcu
Affiliation:Laboratory of Electrochemistry and PATLAB Bucharest, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021, Bucharest, Romania.
Keywords:L(D)-T4, amperometric biosensor, amperometric immunosensor, D-amino acid oxidase, sequential injection analysis, simultaneous detection
Abstract: A sequential injection analysis system was selected for the simultaneous determination of L- and D-T4 in raw material and pharmaceutical products, because of the highest precision and accuracy, the lower consumption of sample and buffer, and the flexibility of selecting different buffers accordingly with the type of sensors utilized as detectors. An amperometric biosensor based on D-amino acid oxidase was used as detector for D-T4 and an immunosensor based on anti-L-T4 was used as detector for L-T4. The SIA/biosensors system can be used reliably on-line in synthesis process control, for the simultaneous assay of L- and D- T4 with a frequency of more than 30 samples per hour.
