Simultaneous Determination of L- and D-T4 Using a Sequential Injection Analysis/Sensors System

Author(s): Raluca-Ioana Stefan van Staden, Jacobus Frederick van Staden, Hassan Y. Aboul-Enein, Ionel Balcu

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 13 , Issue 6 , 2010

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Abstract:

A sequential injection analysis system was selected for the simultaneous determination of L- and D-T4 in raw material and pharmaceutical products, because of the highest precision and accuracy, the lower consumption of sample and buffer, and the flexibility of selecting different buffers accordingly with the type of sensors utilized as detectors. An amperometric biosensor based on D-amino acid oxidase was used as detector for D-T4 and an immunosensor based on anti-L-T4 was used as detector for L-T4. The SIA/biosensors system can be used reliably on-line in synthesis process control, for the simultaneous assay of L- and D- T4 with a frequency of more than 30 samples per hour.

Keywords: L(D)-T4, amperometric biosensor, amperometric immunosensor, D-amino acid oxidase, sequential injection analysis, simultaneous detection

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Article Details

VOLUME: 13
ISSUE: 6
Year: 2010
Page: [497 - 501]
Pages: 5
DOI: 10.2174/138620710791516094
Price: $65

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