Abstract
This paper reviews the development of a magnetic modulation biosensing (MMB) system for rapid, simple and sensitive detection of biological targets in homogeneous solution at low concentrations. It relies on condensation and modulation of the fluorescent-labeled probes attached to magnetic beads using an alternating magnetic field gradient. Condensation of the beads from the entire volume increases the signal while modulation separates the signal from the background noise of the non-magnetized solution. We first discuss the motivation and challenges in specific DNA sequences detection as well as current approaches to overcome some of these challenges. We then present the MMB system, DNA detection schemes and magnetic beads manipulation in solution. Rapid detection at sub-picomolar concentrations of fluorescent-labeled probes as well as of coding sequences of the non-structural Ibaraki virus protein 3 (NS3) complementary DNA (cDNA) without any washing or separation step is also reviewed. Finally, we show preliminary results of protein detection using a ‘sandwich’-based assay.
Keywords: Ibaraki virus, DNA detection, Magnetic modulation, Magnetic beads, Fluorescence and luminescence, Magnetic nanoparticles, FRET
Current Pharmaceutical Biotechnology
Title: Magnetic Modulation Biosensing for Rapid and Homogeneous Detection of Biological Targets at Low Concentrations
Volume: 11 Issue: 1
Author(s): Amos Danielli, Noga Porat, Marcelo Ehrlich and Ady Arie
Affiliation:
Keywords: Ibaraki virus, DNA detection, Magnetic modulation, Magnetic beads, Fluorescence and luminescence, Magnetic nanoparticles, FRET
Abstract: This paper reviews the development of a magnetic modulation biosensing (MMB) system for rapid, simple and sensitive detection of biological targets in homogeneous solution at low concentrations. It relies on condensation and modulation of the fluorescent-labeled probes attached to magnetic beads using an alternating magnetic field gradient. Condensation of the beads from the entire volume increases the signal while modulation separates the signal from the background noise of the non-magnetized solution. We first discuss the motivation and challenges in specific DNA sequences detection as well as current approaches to overcome some of these challenges. We then present the MMB system, DNA detection schemes and magnetic beads manipulation in solution. Rapid detection at sub-picomolar concentrations of fluorescent-labeled probes as well as of coding sequences of the non-structural Ibaraki virus protein 3 (NS3) complementary DNA (cDNA) without any washing or separation step is also reviewed. Finally, we show preliminary results of protein detection using a ‘sandwich’-based assay.
Export Options
About this article
Cite this article as:
Danielli Amos, Porat Noga, Ehrlich Marcelo and Arie Ady, Magnetic Modulation Biosensing for Rapid and Homogeneous Detection of Biological Targets at Low Concentrations, Current Pharmaceutical Biotechnology 2010; 11 (1) . https://dx.doi.org/10.2174/138920110790725375
DOI https://dx.doi.org/10.2174/138920110790725375 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Therapeutic Effects of Micronutrient Supplements on Sperm Parameters: Fact or Fiction?
Current Pharmaceutical Design A Novel Functional Approach Toward Identifying Definitive Drug Targets
Current Medicinal Chemistry Pharmacotherapy of Schizophrenia: The Past, Present and Future
Current Drug Therapy Understanding and Avoiding Antiretroviral Adverse Events
Current Pharmaceutical Design Eosinophilopoiesis at the Cross-Roads of Research on Development, Immunity and Drug Discovery
Current Medicinal Chemistry Porphyrin Metabolisms in Human Skin Commensal Propionibacterium acnes Bacteria: Potential Application to Monitor Human Radiation Risk
Current Medicinal Chemistry NPFF2 Receptor is Involved in the Modulatory Effects of Neuropeptide FF for Macrophage Cell Line
Protein & Peptide Letters The Blood-Central Nervous System Barriers Actively Control Immune Cell Entry into the Central Nervous System
Current Pharmaceutical Design Combination Therapies Using Metformin and/or Valproic Acid in Prostate Cancer: Possible Mechanistic Interactions
Current Cancer Drug Targets Role of Cytokines in Chemotherapy-related Cognitive Impairment of Breast Cancer Patients: A Systematic Review
Current Reviews in Clinical and Experimental Pharmacology Clinical Relevance of the Interaction When Switching Non-Nucleoside Reverse Transcriptase Inhibitors in Patients Infected with HIV
Current Clinical Pharmacology Anti-Obesity Medications in Cancer Therapy: A Comprehensive Insight
Current Cancer Drug Targets Genomic and Genetic Approaches for the Identification of Antifungal Drug Targets
Infectious Disorders - Drug Targets Biosimilars: Current Scientific and Regulatory Considerations
Current Clinical Pharmacology Identification of a Novel Glutathione Conjugate of Diclofenac by LTQOrbitrap
Drug Metabolism Letters A Review of HPLC Methods Used for Determining the Presence of Meloxicam
Current Pharmaceutical Analysis Hemolysis and Rhabdomyolysis after Marathon and Long Distance Running
Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry (Discontinued) Cytokine Expression Profile as Predictive Surrogate Biomarkers for Clinical Events in the Field of Solid Organ Transplantation
Current Protein & Peptide Science Editorial [Hot topic: TGF-β Signaling and its Inhibition: Implication in Fibrosis (Guest Editor: Asish K. Ghosh)]
Current Enzyme Inhibition Evaluation of Insulin Resistance Induced Brain Tissue Dysfunction in Obese Dams and their Neonates: Role of Ipriflavone Amelioration
Combinatorial Chemistry & High Throughput Screening