Recent Developments in Isothermal Titration Calorimetry Label Free Screening

Author(s): William B. Peters, Verna Frasca, Richard K. Brown

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 12 , Issue 8 , 2009


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Abstract:

Isothermal titration calorimetry (ITC) is a label free technique used for direct detection of biological interactions by measurement of the heat given off or taken up during the reaction. In this article we will introduce the ITC technique and review two applications of ITC in drug discovery; small molecule/protein interactions and enzyme kinetics. We will also describe the characteristics of a new miniaturized, ultrasensitive calorimeter. This new microcalorimetry system reduces the quantity of protein (or other macromolecule sample) required to obtain a complete thermodynamic profile (n, K, ΔH and ΔS) by up to 7-fold. The reduction in required sample quantities allows ITC to be effectively utilized at earlier stages of the drug discovery and development process.

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Article Details

VOLUME: 12
ISSUE: 8
Year: 2009
Published on: 01 March, 2012
Page: [772 - 790]
Pages: 19
DOI: 10.2174/138620709789104889
Price: $65

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