Mass Spectrometry in High Throughput Screening: A Case Study on Acetyl-Coenzyme A Carboxylase using RapidFire® – Mass Spectrometry (RF-MS)

Author(s): Maxine Jonas, William A. LaMarr, Can Ozbal

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 12 , Issue 8 , 2009

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Abstract:

In this review various technologies and approaches for the utilization of mass spectrometry in high throughput analyses are discussed. The use of quadrupole-based mass spectrometry in the screening of chemical libraries against enzymatic targets for the identification of inhibitors and/or activators is highlighted. The RapidFire mass spectrometry system, an integrated on-line solid-phase extraction system interfaced to a triple-quadrupole mass spectrometer is described in detail, and the identification of a series of inhibitors of the acetyl-coenzyme A carboxylase (ACC) assay is described.

Keywords: High throughput screening, acetyl-coenzyme A carboxylase, RapidFire, mass spectrometry

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Article Details

VOLUME: 12
ISSUE: 8
Year: 2009
Page: [752 - 759]
Pages: 8
DOI: 10.2174/138620709789104924
Price: $65

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