SPR Imaging for High Throughput, Label-Free Interaction Analysis

Author(s): Christopher Lausted, Zhiyuan Hu, Leroy Hood, Charles T. Campbell

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 12 , Issue 8 , 2009

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Surface plasmon resonance (SPR) sensors have proven themselves over the last 20 years to be an effective method to study biomolecular binding and kinetics without the use of labeling. More recently, the approach has been adapted to high throughput use with the imaging of SPR-active microarrays. This is an excellent tool for monitoring microarray binding in real-time where the microarray probes and targets can include a wide range of molecules. DNA, RNA, antibodies, enzymes, and a range of other proteins have been arrayed and quantitatively analyzed.

Keywords: SPR, surface plasmon resonance imaging, surface plasmon resonance microscopy, microarrays, bioaffinity, antibody screening, kinetics, binding constants, protein arrays, DNA arrays

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Article Details

Year: 2009
Page: [741 - 751]
Pages: 11
DOI: 10.2174/138620709789104933
Price: $65

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