Design and Synthesis of 2-Functionalised Oligonucleotides. Their Application for Covalent Trapping the Protein – DNA Complexes

Author(s): Nina G. Dolinnaya, Eugeny M. Zubin, Elena A. Kubareva, Timofey S. Zatsepin, Tatiana S. Oretskaya.

Journal Name: Current Organic Chemistry

Volume 13 , Issue 11 , 2009

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Abstract:

This review outlines the design and synthesis of oligonucleotides bearing 2-modified residues at predetermined positions within the strand. The relative merits of incorporation of reactive carboxyl, carbonyl, iodoacetamide and disulfide-containing groups into oligonucleotides were considered along with solid-phase synthesis of DNA 2- conjugates. The specific cross-linking of 2-substituted oligonucleotides to nucleic acid-binding proteins (transcription factor NF-κB, restriction-modification enzymes) was shown to be helpful in scanning the protein-DNA interface and studying the conformational dynamics of biopolymer ensembles. The future perspectives of chemically reactive DNA constructs as specific protein decoys are discussed.

Keywords: oligonucleotides, synthesis, Covalent Trapping, Protein, –, DNA, cross-linking

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Article Details

VOLUME: 13
ISSUE: 11
Year: 2009
Page: [1029 - 1049]
Pages: 21
DOI: 10.2174/138527209788680745
Price: $58

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